The aim of the study was to examine how early permanent separation, separation during the day only, or full‐time access to the mother goat affected goat kids during social isolation with a sudden sound of a dog bark at two weeks and two months, and a novel arena test with a novel object at two months. Kids permanently separated reduced their vocalization earlier and had a higher heart rate before and after dog bark during isolation at two weeks, no effect was found on the daytime separated kids. Daytime separated kids bleated more at two weeks and decreased heart rate after dog barking at two months. Daytime separated kids showed the strongest fear reaction in the arena test, no effect was found on the permanently separated kids. Kids separated early vocalized more before novel object and showed more explorative behavior afterwards. Our study shows different responses in goat kids separated early permanent, daytime separated, or kept full‐time with mother, which demonstrates the importance of if and how the mother is present, and the impact of using a wide variety of physiological and behavioral measures when evaluating stress in animal welfare research.